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Sharada AC, Solomon FE, Devi PU, Udupa N, Srinivasan KK.
Acta Oncol. 1996;35(1):95-100
Antitumor and
radiosensitizing effects of withaferin A on mouse Ehrlich ascites carcinoma in
vivo.
Department of Radiobiology, Kasturba Medical College, Manipal, Karnataka,
India.
The antitumor and radiosensitizing effects of withaferin A (WA), a steroidal
lactone from Withania somnifera, was studied on Ehrlich ascites carcinoma in
vivo. The acute LD50(14) for WA in Swiss mice was approximately 80 mg/kg.
Twenty-four hours after i.p. inoculation of 10(6) tumor cells, WA was injected
i.p. at different dose fractions (5 or 7.5 mg/kg x 8, 10 mg/kg x 5, 20 or 30
mg/kg x 2) with or without abdominal gamma irradiation (RT, 75. Gy) after the
first drug dose. Increase in life span and tumor-free survival were studied up
to 120 days. The drug inhibited tumor growth and increased survival, which was
dependent on the WA dose per fraction rather than the total dose. Combination of
RT with all the drug schedules increased tumor cure and tumor-free survival, the
best effect seen after 2 fractions of 30 mg/kg each. In another experiment WA
was given as 2 (40 mg/kg x 2), 3 (30 mg/kg x 3) or 4 (20 mg/kg x 4) fractions at
5, 7 or 10 days after tumor inoculation with or without RT after the first drug
dose. At 7 and 10 days after inoculation the drug was effective only at 40 mg/kg
x 2, but with RT 30 mg/kg x 3 produced an equal effect (20% survival) on 7 day
old tumors.
PMID: 8619948
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