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Fuska J, Prousek J, Rosazza J, Budesinsky M.
Steroids. 1982 Aug;40(2):157-69
Microbial transformations of
natural antitumor agents. 23. Conversion of withaferin-A to 12 beta- and 15
beta-hydroxy derivatives of withaferin-A.
Microbial transformation experiments were conducted with the antitumor
lactone withaferin-A. Cunninghamella elegans NRRL 1393 transformed withaferin-A
(1a) to 15 beta-hydroxywithaferin-A (2a) and 12 beta-hydroxy-withaferin-A (3a).
The hydroxylated metabolites were isolated by solvent extraction and were
purified by column and thin-layer chromatography. Structures of the hydroxylated
metabolites were determined by proton-and carbon-13 NMR, IR and mass spectral
analyses, and by the preparation of acylated derivatives. Compounds 2a and 3a
inhibited the growth and biochemical functions of in vitro grown P-388
lymphocytic leukemic cells.
PMID: 7157453
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