|
Piotrowska H, Jagodzinski PP.
Trichostatin A, sodium butyrate, and 5-aza-2'-deoxycytidine
alter the expression of glucocorticoid receptor alpha and beta
isoforms in Hut-78 T- and Raji B-lymphoma cell lines.
Biomed Pharmacother. 2007 Apr 20
Department of Biochemistry and Molecular Biology, Poznan
University of Medical Sciences, 6 Swiecickiego St., 60-781
Poznan, Poland.
The glucocorticoid receptor (GR) is mainly expressed as
nine-exon alternatively spliced variants, encoding functional
GRalpha and nonfunctional GRbeta. Overexpression of GRbeta
splice variant was found in glucocorticoid-resistant patients
with some autoimmune diseases and hematological malignancies.
Employing reverse transcription, real-time quantitative PCR, and
western blot analysis, we determined an effect of trichostatin A
(TSA), sodium butyrate (NaBu) and 5-aza-2'-deoxycytidine
(5-dAzaC) on GRalpha and GRbeta expression in Hut-78 T- and Raji
B-lymphoma cell lines. We found that TSA, NaBu, and 5-dAzaC
significantly increase the expression of GRalpha transcript and
protein, whereas GRbeta transcript and protein expression was
profoundly decreased in Hut-78 T- and Raji B- lymphoma cell
lines. Our observation suggests that changes of epigenetic
milieu inside cells may alter the expression of GRalpha and
GRbeta isoforms.
PMID: 17498915
|
