Bergmann R.Thiolutin inhibits utilization of glucose and
other carbon sources in cells of Escherichia coli.
Antonie Van Leeuwenhoek. 1989;55(2):143-52
Institut für Allgemeine Botanik, Universität Hamburg, FRG.
Thiolutin was found to inhibit the utilization of glucose and
other growth substrates in Escherichia coli. The inhibition was
detected by a sharp drop of the respiration rate after addition
of the antibiotic. The actual function affected was allocated to
the cytoplasmic membrane of the bacterial cells by the following
evidence: --spheroplasts were affected like intact cells,
--individual reactions of either the electron transport chain or
the glycolytic pathway were not inhibited, --glucose consumption
in the culture stopped and the cells accumulated guanosine
tetraphosphate as under starvation conditions, --activation of
the cell's apo-glucose dehydrogenase restored respiration via
bypassing the glucose phosphotransferase system. It was
concluded that the transport of certain substrates across the
membrane was inhibited.
PMID: 2662903
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