Bioelectrochemistry. 2006 May;68(2):126-33.
A novel biosensor for sterigmatocystin constructed by
multi-walled carbon nanotubes (MWNT) modified with
aflatoxin-detoxifizyme (ADTZ).
Yao DS, Cao H, Wen S, Liu DL, Bai
Y, Zheng WJ.
Lab. of Biotechnology, Life Science and Technology College,
Jinan University, China.
Sterigmatocystin, ST, is carcinogenic mycotoxin with toxicity
second to aflatoxins, contaminated in foods- and feeds-stuff
widely. A three-electrode system was employed to examine the
response character of the covalently united ADTZ-MWNTs electrode
to ST, and the results indicated that an oxidation peak of ST
was observed at about +400 mv, the linear detection range of ST
was from 4.16 x 10(-5) mg/ml (0.13 microM) to 1.33 x 10(-3)
mg/ml (4.29 microM) with the detection limit at 0.13 microM.
Compared to the corresponding results obtained from the MWNTs
modified electrode that ADTZ was directly sediment (adsorbed) on
it, the sensitivity of ours had been improved by two orders of
magnitude, which could provide some important data to further
research.
PMID: 16122991 |
