Cell Biochem Funct. 2007 Sep-Oct;25(5):555-61.
Staurosporine induces tyrosine phosphorylation in
Dictyostelium discoideum proteins.
Andrioli LP, Souza GM, da
Silva AM.
The treatment of cells with staurosporine results in
inhibition and less frequently activation of protein kinases, in
a cell-type specific manner. In the social amoeba Dictyostelium
discoideum, staurosporine induces marked changes in cell
morphology affecting growth and development. Here we describe
that incubation of D. discoideum growing or starved cells with
staurosporine results in a rapid and unexpected tyrosine
phosphorylation on two polypeptides of approximately 64 and
approximately 62 kDa. These proteins emerge as novel substrates
for tyrosine phosphorylation opening up new perspectives for the
study of cell signalling in D. discoideum.
PMID: 16924591 |
