Cutan Ocul Toxicol. 2006;25(2):85-101Intracameral toxicity
of bacterial components muramyl dipeptide and staurosporin:
ciliary cyst formation, epithelial cell apoptosis and
necrosis.
Langford MP, Chen D, Gosslee J, Misra RP, Redens TB,
Texada DE.
Department of Ophthalmology, Louisiana State University
Health Sciences Center, Shreveport, Louisiana 71130-3932, USA.
mlangf@lsuhsc.edu
The uveitogenic bacterial cell wall component muramyl
dipeptide (MDP) is apoptogenic in rabbit kidney cells. The
purpose of this investigation was to assess the cytotoxic
activity of MDP and staurosporin (staurosporin ; induces
cultured corneal and lens cells apoptosis) in rabbit ciliary
body tissue. Anterior uveitis was determined by clinical
symptoms and increased aqueous humor (AH) protein. Ciliary body
tissue was assessed for histological changes, caspase-3
activity, dye uptake, distribution of immunoreactive caspase-3
and DNA ladders at 4 and 6 hours postinjection. Increases in
caspase-3 activity, APOPercentage dye uptake, and localization
of immunoreactive caspase-3 in ciliary epithelial cells were
associated with ciliary cysts of detached nonpigmented
epithelial (NPE) cells, as well as apoptotic and necrotic DNA
ladders in ciliary body tissues from eyes injected with MDP
and/or staurosporin . The results suggest that
intracameral injection of the bacterial components MDP and
staurosporin can induce acute endophthalmic changes
in uveal tissue including formation of ciliary body, NPE and
pigmented epithelial (PE) cell apoptosis, and ciliary body
tissue necrosis.
PMID: 16835145 |
