Neurochem Res. 2006 May;31(5):685-92. Epub 2006 May 23.
staurosporin-induced growth inhibition of glioma cells is
accompanied by altered expression of cyclins, CDKs and CDK
inhibitors.
Harmalkar MN, Shirsat NV.
Neurooncology, Advanced Centre for Treatment, Research and
Education in Cancer (ACTREC), Tata Memorial Centre, Kharghar,
Navi Mumbai 410208, India.
staurosporin was found to bring about complete growth
inhibition of human glioma cell lines. U87 MG cells were
arrested in S phase while U373 MG cells in G2/M phase on
staurosporin treatment. Consistent with this observation, no
change in G1 phase regulators viz., Cyclin D1, D3 and CDK4 was
seen on staurosporin treatment. The levels of CDK2, CDC2,
Cyclin A and Cyclin B proteins decreased, while the levels of
CDK inhibitors viz., p21 and p27 were found to increase on
staurosporin treatment. The mRNA levels of CDK2 and CDC2 genes
were also found to decrease on staurosporin treatment. Thus
apart from staurosporin's known direct inhibitory effect on
CDK2 and CDC2 activities, staurosporin was found to
down-regulate activities of these two kinases by modulating the
expression of the kinases themselves as well that of their
activating partners (Cyclins) and their inhibitors.
PMID: 16770740 |
