Anal Chem. 2006 Jul 1;78(13):4624-9.
LC-MS/MS screen for penitrem A and roquefortine C in serum
and urine samples.
Tor ER, Puschner B, Filigenzi MS, Tiwary AK,
Poppenga RH.
California Animal Health and Food Safety Laboratory System,
Toxicology Laboratory, University of California, Davis,
California 95616, USA.
ertor@ucdavis.edu
A rapid LC-MS/MS method, using a triple quadrupole/linear ion
trap mass spectrometer, was developed for determination of
penitrem A and roquefortine C in serum and urine samples.
Penitrem A and roquefortine C were extracted from samples with
methylene chloride. The extracts were injected onto a liquid
chromatograph coupled with a hybrid triple quadrupole/linear ion
trap mass spectrometer. Seven replicate fortifications of serum
at 0.001 microg/g (1 ppb) each of penitrem A and roquefortine C
gave average recoveries of 90% with 10% CV (relative standard
deviation) and 97% with 3% CV, respectively. Seven replicate
fortifications of urine at 0.001 microg/g (1 ppb) each of
penitrem A and roquefortine C gave average recoveries of 98%
with 12% CV and 100% with 6% CV, respectively. This is the first
report of a positive mass spectrometric identification and
quantitation of both compounds in urine and serum samples from
dog intoxication cases.
PMID: 16808474 |
