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McLaughlin J, Lambert D, Padfield PJ, Burt JP, O'Neill CA.
The mycotoxin patulin, modulates tight junctions in caco-2 cells.
Toxicol In Vitro. 2009 Feb;23(1):83-9.
University of Manchester, Faculty of Medicine and Health Sciences, Section of
Gastrointestinal Sciences, Stopford Building, Oxford Road, Manchester M13 9PT,
UK.
The mycotoxin patulin is a common contaminant of fruit. Here, we demonstrate
that patulin reduces the barrier properties of the intestinal cell line, caco-2
by specific effects on tight junction components. Within 5h of exposure to
100muM toxin, the transepithelial electrical resistance of caco-2 monolayers was
reduced by approximately 95% and the monolayer became more permeable to
FITC-labelled dextrans of 4-40kDa. Immunoblotting revealed occludin proteolysis
and a significant reduction in ZO-1 levels. Patulin had no influence on claudin
levels but marked changes in their distribution were observed. These data
indicate that patulin decreases the barrier properties of caco-2 monolayers by
modulation of the tight junction.
PMID: 19013514
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