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Tettamanti G, Malagoli D, Marchesini E, Congiu T, de Eguileor
M, Ottaviani E.
Oligomycin A induces autophagy in the IPLB-LdFB insect cell
line.
Cell Tissue Res. 2006 Oct;326(1):179-86. Department of
Structural and Functional Biology, University of Insubria,
21100, Varese, Italy.
Functional and morphological modifications in the IPLB-LdFB
insect cell line were examined following a short treatment with
a reversible inhibitor of mitochondrial ATP synthase, oligomycin
A, and subsequent incubation for various times in
oligomycin-A-free medium. Oncosis, apoptosis and autophagy at
variable percentages were observed under the various
experimental conditions. Together with oncotic and apoptotic
pathways that lead directly to cell death, the insect cells
responded to ATP depletion with autophagy. Our results revealed
that, in most cases, autophagy failed to restore cellular
homeostasis, probably because of a massive sequestration of
mitochondria in autophagic vacuoles. This critical event was a
"point of no return" and ultimately resulted in cell necrosis.
However, cells with a misshapen body and nucleus resembling
"resistant forms" were observed at the end of the experiments.
Our findings indicate that oligomycin-A-induced autophagy can
promote cell protection or cell destruction and is an open-ended
process that can lead to survival or death depending on a
combination of concomitant factors.
PMID: 16767407
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