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Yoshizawa T, Kohno H, Ikeda K, Shinoda T, Yokohama H, Morita K,
Kusada O, Kobayashi Y. Biosci Biotechnol Biochem. 2004
Oct;68(10):2076-85.
A practical method for measuring deoxynivalenol, nivalenol,
and T-2 + HT-2 toxin in foods by an enzyme-linked immunosorbent
assay using monoclonal antibodies.
Department of Biochemistry and Food Science, Faculty of
Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan.
We have developed and tested an enzyme-linked immunosorbent
assay system for individual measurement of deoxynivalenol,
nivalenol, and T-2 + HT-2 toxin using monoclonal antibodies for
3,4,15-triacetyl-nivalenol, for both 3,4,15-triacetyl-nivalenol
and 3,15-diacetyl-deoxynivalenol, and for acetyl-T-2 toxin. The
assay system comprised three kits (desinated the DON + NIV kit,
the NIV kit, and the T-2 + HT-2 kit). The practical performance
of the enzyme-linked immunosorbent assay system was assessed by
assaying trichothecene mycotoxins in wheat kernels. The
enzyme-linked immunosorbent assay system meets all the
requirements for use in a routine assay in terms of sensitivity
(detection limit: deoxynivalenol 80 ng/g, nivalenol 80 ng/g, T-2
toxin 30 ng/g), reproducibility (total coefficient of variation:
1.9-6.2%), accuracy (recovery: 93.8-112.0%), simplicity and
rapidity (time required: <2 h), mass handling (>42
samples/assay), and a good correlation with gas
chromatography-mass spectrometry (r=0.9146-0.9991). Components
derived from the wheat extract did not interfere with the assay
kits. The enzyme-linked immunosorbent assay system is a useful
alternative method to gas chromatography-mass spectrometry,
liquid chromatography-mass spectrometry, or liquid
chromatography-ultraviolet absorption for screening cereals and
foods for trichothecene mycotoxin contamination.
PMID: 15502352
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