Kristensen R, Berdal KG, Holst-Jensen
A.Simultaneous detection and identification of
trichothecene- and moniliformin
- producing
Fusarium species based on multiplex SNP analysis.
J Appl Microbiol. 2007 Apr;102(4):1071-81.
Section of Feed and Food Microbiology, National Veterinary
Institute, Oslo, Norway.
AIM: To develop a multiplex identification method for
trichothecene- and moniliformin-producing Fusarium species.
METHOD AND RESULTS: In this article, we present a single
nucleotide polymorphism (SNP) assay to simultaneously detect and
identify 16 trichothecene- and moniliformin-producing Fusarium
species. A number of SNP primers are designed to detect clades
of species with particular mycotoxigenic synthetic abilities.
The assay is based on minisequencing using SNaPshot reactions
and the SNP primers are designed based on motifs derived from
phylogenetic analyses of translation elongation factor-1alpha
sequences. The present version of the Fusarium SNP assay can
distinguish major groups of trichothecene producers; the
strict-type-A, the strict-type-B, the type-A and type-B
trichothecene producers and the putative moniliformin producers.
The SNP assay was validated against five naturally infected
cereal samples that previously had been analysed
morphologically, chemically and by a multiplex DNA array
hybridization. CONCLUSIONS: The Fusarium SNP assay reveals the
advantages of using SNPs for multiplex species identification.
Significance AND IMPACT OF THE STUDY: The current assay may
qualify as a high-throughput screening method for small-grain
cereals in the feed and food chain, and may facilitate detection
of new or introduced Fusarium species.
|
