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Quarta A, Mita G, Haidukowski M, Logrieco A, Mule G, Visconti
A.
Multiplex PCR assay for the identification
of nivalenol, 3- and 15-acetyl-
Vomitoxin (deoxynivalenol) (15A DON)
chemotypes in Fusarium.
FEMS Microbiol Lett. 2006 Jun;259(1):7-13.
Institute of Sciences of Food Production, ISPA, National
Research Council, CNR, Lecce, Italy.
The ability to rapidly distinguish trichothecene chemotypes in a
given species/population of the genus Fusarium is important due
to significant differences in the toxicity of these secondary
metabolites. A multiplex PCR assay, based on primer pairs
derived from the Tri3, Tri5 and Tri7 genes of the trichothecene
gene cluster was established for the identification of the
different chemotypes among Fusarium graminearum, F. culmorum and
F. cerealis. Using the selected primers, specific amplification
products of 625, 354 and 708 bp were obtained from Fusarium
isolates producing nivalenol, 3-acetyl-deoxynivalenol and
15-acetyl-deoxynivalenol, respectively. Moreover, the multiplex
PCR was successfully used to identify the chemotype of the
Fusarium species contaminating wheat kernels. Four picograms of
fungal DNA were found to be necessary to obtain a visible
amplification product.
PMID: 16684095
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