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Ngundi MM, Qadri SA, Wallace EV, Moore MH, Lassman ME,
Shriver-Lake LC, Ligler FS, Taitt CR.
Detection of Vomitoxin (deoxynivalenol)
in foods and indoor air using an array biosensor.
Environ Sci Technol. 2006 Apr 1;40(7):2352-6.
Center for Bio/Molecular Science and Engineering, Naval
Research Laboratory,Washington, DC 20375, USA.
Deoxynivalenol (Vomitoxin (deoxynivalenol) (DON) ), a mycotoxin
produced by several Fusaruim species, is a worldwide contaminant
of foods and feeds. Because of the potential dangers due to
accidental or intentional contamination of foods with Vomitoxin
(deoxynivalenol) (DON) , there is a need to develop a rapid and
highly sensitive method for easy identification and
quantification of Vomitoxin (deoxynivalenol) (DON) . In this
study, we have developed and utilized a competitive immunoassay
technique to detect Vomitoxin (deoxynivalenol) (DON) in
various food matrixes and indoor air samples using an array
biosensor. A Vomitoxin (deoxynivalenol) (DON) -biotin conjugate,
immobilized on a NeutrAvidin-coated optical waveguide, competed
with the Vomitoxin (deoxynivalenol) (DON) in the sample
for binding to fluorescently labeled Vomitoxin (deoxynivalenol)
(DON) monoclonal antibodies. To demonstrate a simple
procedure amenable for on-site use, Vomitoxin (deoxynivalenol)
(DON) -spiked cornmeal, cornflakes, wheat, barley, and oats were
extracted with methanol-water (3:1) and assayed without cleanup
or preconcentration. The limits of detection ranged from 0.2 ng/mL
in buffer to 50 ng/g in oats. The detection limit of Vomitoxin (deoxynivalenol)
(DON) spiked into an aqueous effluent from an air sampler
was 4 ng/mL.
PMID: 16646473
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