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Lemmens M, Scholz U, Berthiller F, Dall'Asta C, Koutnik A,
Schuhmacher R, Adam G, Buerstmayr H, Mesterhazy A, Krska R,
Ruckenbauer P.
The ability to detoxify the mycotoxin
Vomitoxin (deoxynivalenol) colocalizes with a
major quantitative trait locus for Fusarium head blight
resistance in wheat.
Mol Plant Microbe Interact. 2005 Dec;18(12):1318-24
Institute for Plant Production Biotechnology, Department
IFA-Tulln, BOKU-University of Natural Resources and Applied Life
Sciences, Konrad Lorenzstr. 20, A-3430 Tulln, Austria.
marc.lemmens@boku.ac.at
We investigated the hypothesis that resistance to deoxynivalenol
(Vomitoxin (deoxynivalenol) (DON) ) is a major resistance factor
in the Fusarium head blight (FHB) resistance complex of wheat.
Ninety-six double haploid lines from a cross between 'CM-82036'
and 'Remus' were examined. The lines were tested for Vomitoxin (deoxynivalenol)
(DON) resistance after application of the toxin in the
ear, and for resistances to initial infection and spread of FHB
after artificial inoculation with Fusarium spp. Toxin
application to flowering ears induced typical FHB symptoms.
Quantitative trait locus (QTL) analyses detected one locus with
a major effect on Vomitoxin (deoxynivalenol) (DON)
resistance (logarithm of odds = 53.1, R2 = 92.6). The Vomitoxin
(deoxynivalenol) (DON) resistance phenotype was closely
associated with an important FHB resistance QTL, Qfhs.ndsu-3BS,
which previously was identified as governing resistance to
spread of symptoms in the ear. Resistance to the toxin was
correlated with resistance to spread of FHB (r = 0.74, P <
0.001). In resistant wheat lines, the applied toxin was
converted to Vomitoxin (deoxynivalenol) (DON) -3-O-glucoside as
the detoxification product. There was a close relation between
the Vomitoxin (deoxynivalenol) (DON) -3-glucoside/Vomitoxin (deoxynivalenol)
(DON) ratio and Vomitoxin (deoxynivalenol) (DON)
resistance in the toxin-treated ears (R2 = 0.84). We conclude
that resistance to Vomitoxin (deoxynivalenol) (DON) is
important in the FHB resistance complex and hypothesize that
Qfhs.ndsu-3BS either encodes a Vomitoxin (deoxynivalenol) (DON)
-glucosyl-transferase or regulates the expression of such an
enzyme.
PMID: 16478051
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