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Gouze ME, Laffitte J, Dedieu G, Galinier A, Thouvenot JP,
Oswald IP, Galtier P.
Individual and combined effects of low
oral doses of Vomitoxin (deoxynivalenol) (DON)and nivalenol in
mice.
Cell Mol Biol (Noisy-le-grand). 2005 Nov 4;51 Suppl:OL809-17
Laboratoire de Pharmacologie-Toxicologie, INRA, 180 Chemin de
Tournefeuille, 31931 Toulouse, France.
Deoxynivalenol (DON) and nivalenol (NIV) are toxic Fusarium
secondary trichothecene metabolites that often co-occur
regularly in cereal grains. These compounds were compared for
their toxicity towards C57BL/6 mice on several parameters
including alteration in plasma biochemistry, immune system
reactivity and hepatic drug metabolism capacity. Mice received
individual or combined oral doses of each toxin: 0.071 or 0.355
mg/kg of body weight, administrated three days a week for 4
weeks. Food consumption was altered by the single administration
of 0.355 mg/kg of NIV, although no noticeable change of body and
organ weights or liver protein contents was detected. NIV
administration did cause also significant changes in total CO2
and uric acid concentrations in plasma. Individual toxin
exposures led to increases in plasma IgA without no detectable
change in the ex vivo production of cytokine by splenocytes. The
liver ethoxyresorufin O-deealkylase, pentoxyresorufin O-depenthylase
and glutathione S-transferase activities were increased in
concert with cytochrome P4501a and P4502b subfamily expression.
Administration of combinations of DON and NIV resulted in
responses similar to that observed using individual doses of
each toxin. However, depending on the ratio of toxin doses and
biochemical parameters, some responses could be also additive
(plasma IgA and hepatic DCNB conjugation) or synergistic (plasma
uric acid).
PMID: 16375817
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