Cetin Y, Bullerman LB.
Confirmation of reduced toxicity of
Vomitoxin (deoxynivalenol) (DON) in
extrusion-processed corn grits by the MTT bioassay.
J Agric Food Chem. 2006 Mar 8;54(5):1949-55.
Department of Food Science and Technology, 143 Filley Hall,
University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0919,
USA.
The objective of this study was to determine the loss of
toxicity of deoxynivalenol in extruded cereal-based products by
the tetrazolium salt (MTT) bioassay using a sensitive Chinese
hamster ovary (CHO-K1) cell line and to compare the results to
chemical (high-performance liquid chromatography, HPLC) and
biochemical (enzyme-linked immunosorbant assay, ELISA) methods
of analysis. A split-split plot design was used for the
extrusion process experiments at temperatures of 150, 175, and
200 degrees C and screw speeds of 70 and 140 rpm. The initial
mean deoxynivalenol concentration in the corn grits artificially
contaminated with Fusarium graminearum was found to be 23.5
mug/g as measured by HPLC. The percent reductions of
deoxynivalenol in the contaminated corn grits upon extrusion
processing ranged from 22 to 35%, from 21 to 34%, and from 21 to
37% as measured by HPLC, ELISA, and MTT bioassay, respectively.
The MTT bioassay results were more closely correlated with HPLC
(r = 0.90) results than with ELISA results (r = 0.78). The MTT
bioassay, using a sensitive mammalian cell line, was
demonstrated to be a useful method for quantification of
deoxynivalenol as well as a potential toxicity screening method
for contaminated extruded cereal-based products.
PMID: 16506858
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