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Bretz M, Beyer M, Cramer B, Humpf HU.
Stable isotope dilution analysis of the Fusarium
mycotoxins Vomitoxin (deoxynivalenol) (DON) and 3-acetyl
Vomitoxin (deoxynivalenol)
(DON) .
Mol Nutr Food Res. 2006 Mar;50(3):251-60
Institut für Lebensmittelchemie, Westfälische
Wilhelms-Universität Münster, Germany.
Trichothecenes are secondary metabolites produced by several
fungi of the Fusarium genus during their growth period. They
inhibit protein biosynthesis in eukaryotic cells resulting in
numerous toxic effects such as diarrhea, vomiting, and
gastro-intestinal inflammation. Considering its occurrence in
food and feedstuff, deoxynivalenol (DON) is one of the most
important trichothecenes. We report the synthesis of stable
isotope labeled 15-d(1)-deoxynivalenol (15-d(1)-DON) from its
natural precursor 3-acetyldeoxynivalenol (3-AcDON) as starting
material. Furthermore, a method for the analysis of DON and
3-AcDON using HPLC-MS/MS with stable isotope labeled 15-d(1)-DON
and 3-d(3)-AcDON as internal standards has been developed. In
total, 18 cereal product samples were analyzed with
contamination levels ranging from 10-301 microg/kg for DON and
5-14 microg/kg for 3-AcDON. This is the first report of an
isotope dilution MS method for the analysis of type B-trichothecenes.
PMID: 16521158 |
