Bretz M, Beyer M, Cramer B, Humpf HU.
Stable isotope dilution analysis of the
Fusarium mycotoxins Vomitoxin (deoxynivalenol) and
3-acetyl Vomitoxin (deoxynivalenol)
Mol Nutr Food Res. 2006 Mar;50(3):251-60
Institut für Lebensmittelchemie, Westfälische
Wilhelms-Universität Münster, Germany.
Trichothecenes are secondary metabolites produced by several
fungi of the Fusarium genus during their growth period. They
inhibit protein biosynthesis in eukaryotic cells resulting in
numerous toxic effects such as diarrhea, vomiting, and
gastro-intestinal inflammation. Considering its occurrence in
food and feedstuff, deoxynivalenol (Vomitoxin (deoxynivalenol)
(DON) ) is one of the most important trichothecenes. We report
the synthesis of stable isotope labeled 15-d(1)-deoxynivalenol
(15-d(1)-Vomitoxin (deoxynivalenol) (DON) ) from its natural
precursor 3-acetyldeoxynivalenol (3-AcVomitoxin (deoxynivalenol)
(DON) ) as starting material. Furthermore, a method for the
analysis of Vomitoxin (deoxynivalenol) (DON) and
3-AcVomitoxin (deoxynivalenol) (DON) using HPLC-MS/MS with
stable isotope labeled 15-d(1)-Vomitoxin (deoxynivalenol) (DON)
and 3-d(3)-AcVomitoxin (deoxynivalenol) (DON) as internal
standards has been developed. In total, 18 cereal product
samples were analyzed with contamination levels ranging from
10-301 microg/kg for Vomitoxin (deoxynivalenol) (DON) and
5-14 microg/kg for 3-AcVomitoxin (deoxynivalenol) (DON) . This
is the first report of an isotope dilution MS method for the
analysis of type B-trichothecenes.
PMID: 16521158
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