Ikewaki N, Yamada A, Inoko H.Depolymerization of actin
filament by cytochalasin E induces interleukin-8 production and
up-regulates CD54 in the HeLa epithelial cell line.
Microbiol Immunol. 2003;47(10):775-83.
Division of Immunology, Kyushu University of Health and
Welfare, Faculty of Health and Science, Nobeoka, Miyazaki
882-8508, Japan.
We previously reported that the depolymerization of actin
filament by cytochalasin E enhances low affinity Fcepsilon
receptor II (CD23) expression on the human monocyte-like cell
line, U937 (J. Clin. Immunol. 20: 235, 2000). In this study, we
found that cytochalasin E strongly induces interleukin-8 through
an epithelial cell line, HeLa, in dose- and time-dependent
manners as assessed by enzyme-linked immunoassay and reverse
transcription-polymerase chain reaction techniques. In addition,
interleukin-8 production in the HeLa cells cultured with
cytochalasin E was blocked in the presence of protein kinase C
inhibitors, Go6976 and H-7. On the other hand, it was found that
CD54 (intercellular adhesion molecule-1; ICAM-1) expression on
the HeLa cells and the secretion of soluble CD54 were
significantly up-regulated after culturing with cytochalasin E,
and that these up-regulations of CD54 were also suppressed by
Go6976. Taken together, these findings indicate that
cytochalasin E activates protein kinase C under the
depolymerization of actin filament, leading to the induction of
interleukin-8 production and the up-regulation of CD54 in HeLa
cells.
PMID: 14605444 |
