Hansson KM, Tengvall P, Lundstrom I, Ranby M, Lindahl TL.
Comparative studies with surface plasmon resonance and free
oscillation rheometry on the inhibition of platelets with
cytochalasin E and monoclonal antibodies towards GPIIb/IIIa.
Biosens Bioelectron. 2002 Sep;17(9):761-71.
Department of Biomedicine and Surgery, Division of Clinical
Chemistry, Linköping University Hospital, SE-581 85, Linköping,
Sweden.
kenny.m.hansson@astrazeneca.com
In the haemostatic system a multitude of processes are
intertwined in fine-tuned interactions that arrest bleeding,
keep the circulatory system open, and the blood flowing. The
occurrence of both surface and bulk interactions adds an
additional dimension of complexity. These insights have led to
the belief that global overall procedures can inform on the
likely behaviour of the system in health and disease. Two
sensing procedures: surface plasmon resonance (SPR), which
senses surface interactions, and free oscillation rheometry
(FOR), which senses interactions within the bulk, have been
combined and evaluated. The contribution of blood cells, mainly
platelets, to the SPR and FOR signals was explored by
simultaneous SPR and FOR measurement during native whole blood
coagulation, accelerated via the platelets through addition of
SFLLRN peptide and inhibition of platelet aggregation with
abciximab (ReoPro) and of shape change with cytochalasin E. The
SPR technique was found to be sensitive to inhibition of blood
cell functions such as adhesion to and spreading on surfaces, as
well as platelet aggregation. SPR seemed not to be directly
sensitive to fibrin polymerisation in coagulating whole blood.
The FOR technique detected the coagulation as a bulk phenomenon,
i.e. the gelation of the blood due to fibrin formation was
detected. The combination of SPR and FOR may therefore be
suitable for studies on blood cell functions during coagulation.
PMID: 12191924 |
