Linnett PE, Mitchell AD, Osselton MD, Mulheirn LJ, Beechey RB.
Citreoviridin, a specific inhibitor of the mitochondiral
adenosine triphosphatase.
Biochem J. 1978 Mar 15;170(3):503-10.
- Citreoviridin was a potent inhibitor of the soluble
mitochondrial ATPase (adenosine triphosphatase) similar to
the closely related aurovertins B and D.
- Citreoviridin inhibited the following mitochondrial
energy-linked reactions also: ADP-stimulated respiration in
whole mitochondria from ox heart and rat liver; ATP-driven
reduction of NAD+ by succinate; ATP-driven NAD
transhydrogenase and ATPase from ox heart submitochondrial
particles.
- The dissociation constant (KD) calculated by a simple
law-of-mass-action treatment for the citreoviridin--ATPase
complex was 0.5--4.2micron for ox-heart mitochondrial
preparations and 0.15micron for rat liver mitochondria.
- Monoacetylation of citreoviridin decreased its
inhibitory potency (KD=2--25micron, ox heart; KD=0.7micron,
rat liver). Diacetylation greatly decreased the inhibitory
potency (KD=60--215micron, ox heart).
- Hydrogenation of citreoviridin monoacetate diminished
its inhibitory potency considerably. 6. No significant
enhancement of fluorescence was observed when citreoviridin
interacted with the mitochondrial ATPase.
PMID: 148274 |
