Wang WQ, Zhao XY, Gong XB, Zhang XHCerulenin changes
apoptosis related genes expression in multiple myeloma cell line
U266
The Hematology Department, Second Affiliated Hospital of
Medical School, Zhejiang University, Hangzhou 310009, China.
To study the expression changes of apoptosis related genes
induced by cerulenin in multiple myeloma cell line U266 and
explore its molecular mechanism. METHODS: The expression changes
of 96 apoptosis related genes were analyzed by superArray cDNA
in U266 cells treated with cerulenin (20 microg/ml) for 12 h.
Semi-quantitive RT-PCR was used to confirm the representative
expression changes genes, Rip2, caspase9 and TRAF2. RESULTS:
After treated with cerulenin for 12 h, 44 apoptosis related
genes expression in the U266 cells were changed, among which 41
were over 2 fold increase and 3 over 2 fold decrease. The
expression of caspase 9 was increased markedly, indicating that
mitochondria pathway played a key role in cerulenin inducing
apoptosis and TRAF2 expression change suggested that nuclear
factor (NF) participates in cerulenin inducing apoptosis.
CONCLUSION: The death acceptor signaling pathway and the death
acceptor non-dependence signaling pathway co-regulate cerulenin
inducing apoptosis in U266 cells. Mitochondria pathway played
the key role and nuclear factor (NF) participates in the
apoptosis process.
PMID: 17877200 |
