Salles FT, Hespanhol AM, Jaeger RG, Marques MM.
Brefeldin-A induces apoptosis in human adenoid cystic carcinoma
cultured cells.
Oral Oncol. 2004 Jul;40(6):585-90
Department of Oral Pathology, School of Dentistry, University of
São Paulo, Av. Prof. Lineu Prestes 2227, Cidade Universitária,
CEP 05508 900 São Paulo-SP, Brazil. fsalles@usp.br
Adenoid cystic carcinoma (ACC) of salivary glands is
characterized by a high rate of local recurrences, neurotropism
and metastasis. ACC long-term survival rate is not promising.
Thus, different chemotherapeutical approaches had been proposed
for this neoplasm, including apoptosis induction by different
drugs. This work evaluates the efficacy of Brefeldin-A (BFA), a
potent apoptosis inducer, on ACC cultured cells (CAC2 cell
line). CAC2 cells were treated with a 375 microM BFA solution in
serum-free medium during 18 h. CAC2 cells grown in DMEM
supplemented with 10% fetal bovine serum served as controls.
Apoptotic cell recognition and counting were carried out through
Hoechst staining. Transmission electron microscopy and
immunofluorescence assessed the effect of BFA on CAC2 cells
phenotype. Treated cultures showed a high apoptotic index
presenting +/-30% of cells in evident apoptosis, when compared
to controls. Apoptotic CAC2 cells also exhibited different
alterations such as cytoplasmic vesicles formation and
mitochondrial changes. Cultured ACC cells are strongly
susceptible to apoptosis induction under BFA treatment, which
may constitute a promising tool in further chemotherapeutical
approaches.
PMID: 15063386
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