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Freeman FM, Rose SP, Scholey AB.
Two time windows of
anisomycin-induced amnesia for passive avoidance training in the day-old chick.
Neurobiol Learn Mem. 1995 May;63(3):291-5.
The antibiotic anisomycin (ANI), a protein synthesis
inhibitor, was used to investigate the time-related changes in
protein synthesis following passive avoidance training in the
day-old chick. Retention of memory for this simple learning task
is known to be prevented by protein synthesis inhibitors within
the first hour post-training. Here we report a second, later
time window during which inhibition of protein synthesis results
in amnesia following one-trial passive avoidance training. Birds
were given bilateral intracranial injections of ANI (10
microliters/hemisphere of a 30 mM solution) at various times
relative to training and tested 24 h later. Injections given
between 0.5 h prior to 1.5 h post-training or 4-5 h
post-training, but not at later or at intervening times,
resulted in amnesia. These results are discussed in the context
of earlier findings, using the inhibitor of glycoprotein
synthesis 2-deoxygalactose, that memory formation shows two
glycoprotein-synthesis-dependent periods of sensitivity
(Scholey, Rose, Zamani, Bock, & Schachner, 1993). The time
windows of susceptibility of ANI and 2-Dgal are consistent with
a model in which there are two waves of neural activity
following training; during the second, commencing 4 h after
training, proteins are synthesized and then glycosylated as part
of the establishment of an enduring memory trace.
PMID: 7670843
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