Bonnafous JC, Dornand J, Mani JC.
Alamethicin or detergent permeabilization of the cell
membrane as a tool for adenylate cyclase determination.
Biochim Biophys Acta. 1982 Jun 8;720(3):235-41.
Treatment of mouse lymphocytes with very low concentrations
of alamethicin or Lubrol PX induces spontaneous permeabilization
of the plasma membrane to ATP and allows determination of
adenylate cyclase activity in whole cells. The permeabilized
cells retain responsiveness to hormones (isoproterenol,
adenosine analogs) and to fluoride. The main advantage of this
new method is that it does not require any homogenization step,
and thus adenylate cyclase activities can be accurately and
reproducibly measured with very low amounts of cells. It should
be especially useful for the study of purified lymphocyte
subpopulations.
PMID: 6285993
|
