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Huang XH, Zhang XH, Li YH, Wang JL, Yan X, Xing LX, Wang FR
Experimental lung carcinogenic in vivo study of aflatoxin G1 in NIH mice
Zhonghua Bing Li Xue Za Zhi. 2004 Jun;33(3):260-3
Lab of Experimental Pathology, Hebei Medical University,
Shijiazhuang 050017, China.Aflatoxin G1 is a member of
the carcinogenic aflatoxin family produced by aspergillus
flavus. It is a major contaminating mycotoxin in food in areas
of China with high cancer incidence. The purpose of this study
is to explore the carcinogenic effects of Aflatoxin G1 in NIH
mice. METHODS: NIH mice were randomly divided into three groups.
Two experimental groups were treated intragastrically by gavage
with Aflatoxin G1 3 microg/kg and Aflatoxin G1 30 microg/kg
respectively, 3 times a week for 24 weeks. The control group was
treated with normal saline. All mice were fed with food that was
free of AFGs as confirmed by HPLC analysis. The mice were
weighed every week throughout the entire experiment, and then
sacrificed and examined pathologically at the 58th and 74th
weeks respectively. RESULTS: Compared with control mice
receiving no Aflatoxin G1, bronchial epithelial hyperplasia,
alveolar hyperplasia and adenocarcinoma of lung were observed in
mice receiving Aflatoxin G1 treatment. The incidences of
bronchial epithelial hyperplasia, alveolar hyperplasia and
adenocarcinoma of lung were 60.0%, 10.0% and 30.0% for mice
receiving 3 microg/kg Aflatoxin G1 and 28.6%, 35.7%, 42.9% for
mice receiving 30 microg/kg of the toxin, respectively.
CONCLUSION: Oral administration of Aflatoxin G1 can induce
hyperplastic lesions and adenocarcinoma of lung in NIH mice.
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