Groopman JD, Fowler KW, Busby WF Jr, Wogan GN.Interaction of
aflatoxin B2 with rat liver DNA and histones in vivo
Carcinogenesis. 1981;2(12):1371-3.
The interaction of aflatoxin B2 (AFB2) in vivo with rat liver
nuclear macromolecules was examined in an attempt to correlate
this binding with biological potency. The incorporation of
[3H]AFB2 residues into rat liver histones and DNA was determined
2, 24 and 48 h following administration of a single i.p. dose of
1 mg [3H]AFB2/kg body weight. At each time point, histone H1 and
the total histone fraction contained 5--30-fold more [3H]AFB2
moieties than did DNA on a weight basis. Analytical
reversed-phase h.p.l.c. of the acid hydrolysis products
resulting from AFB2 binding to DNA revealed that 85% of the
radioactivity co-chromatographed with the major aflatoxin B1-DNA
adduct, 2,3-dihydro-2-(N7-guanyl)-3-hydroxyaflatoxin B1. These
studies revealed an apparent correlation between AFB2 derived
binding to DNA in vivo in rats and its potency as a toxin and
carcinogen in this species.
PMID: 7326836
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