Martins HM, Mendes Guerra MM, d'Almeida Bernardo FM.
Occurrence of aflatoxin B1 in dairy cow feed over 10 years
in Portugal (1995-2004).
Rev Iberoam Micol. 2007 Mar;24(1):69-71.
Laboratório Nacional de Investigação Veterinária, Serviço de
Micologia, Estrada de Benfica, 701, Lisboa, Portugal.
marina.martins@lniv.min-agricultura.pt
In Portugal, there is rather little information about the
natural occurrence of aflatoxin in feedstuffs. The aim of this
work was to report the results of screening the natural
incidence of aflatoxin B1 in samples of cattle feed collected
from seven dairy cow's farms from Portugal distributed in
several locations of the country. One thousand and one samples
were taken from 1995 to 2004. High performance liquid
chromatography (HPLC) was used for separation, identification
and quantification of the compound. Detection limit was 1 microg/kg.
Aflatoxin B1 was detected in 374 (37.4%) of the samples. The
incidence and mean content of Aflatoxin B1 was generally low.
Levels of aflatoxin B1 above the maximum limit established in
Portugal (5 microg/kg) for dairy cattle feed samples were
observed in 62 samples (6.2%) with levels ranging from 5.1 to 74
microg/kg. Out of those 62 samples, 3.7% had levels between 5.1
to 10 (mean 7.8); 1.8% had a contamination level of 10.1 to 20
(mean 12.0), and 0.7% exceeded 20.1 microg/kg (mean 50.4). On
the last two years (2003-04) none of the samples exceeded the
maximum permissible level of the toxin.
PMID: 17592898
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