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Zinedine A, Gonzalez-Osnaya L, Soriano JM, Molto JC, Idrissi
L, Manes J.
Presence of aflatoxin M1 in pasteurized
milk from Morocco.
Int J Food Microbiol. 2007 Feb 28;114(1):25-9.
Laboratory of Food Toxicology, National Institute of Health,
27 Avenue Ibn Batouta, PO Box 769 Agdal, Rabat, Morocco.
zinedineab@yahoo.fr
Fifty four samples of pasteurized milk produced by five
different dairies from Morocco were surveyed for the presence of
aflatoxin M1 (Aflatoxin M1 1) using immunoaffinity columns and
liquid chromatography coupled to fluorescence detection.
Confirmation of Aflatoxin M1 identity in positive samples was
based on the formation of Aflatoxin M1 hemi-acetal derivative
(Aflatoxin M1 2a) after derivatization with trifluoracetic acid.
Analytical results showed that 88.8% of the samples were
contaminated with Aflatoxin M1 1; 7.4% being above the maximum
level of 0.05 microg/L set by the Moroccan and European
regulations for Aflatoxin M1 in liquid milk. The incidence of
Aflatoxin M1 in milk from these dairies was 100, 92.3, 90, 83.3
and 77.7% respectively, with Aflatoxin M1 levels ranging from
0.001 to 0.117 microg/L and a mean value of 0.0186 microg/L.
Based on the results presented in this study, the estimated
daily intake of Aflatoxin M1 was 3.26 ng/person/day. In this
work, data on the natural occurrence of Aflatoxin M1 in
pasteurized milk produced in Morocco is presented for the first
time.
PMID: 17175055
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