Effect of Nivalenol on IL-1beta and TNF-alpha secretion
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2010 Apr;26(4):313-215.
Cao PH, Cao JL, Cao LM, Yang YJ, Li WB.
Department of Clinical Laboratory Medicine, Xi'an Medical
University, Xi'an
710021, China.
AIM: To investigate the effect of Nivalenol ( NIV) and
Selenium(Se) on the levels of IL-1beta
and TNF-alpha in the cultured chondrocytes.
METHODS: Human chondrocytes
cultured in vitro were treated with or without NIV and Se. The
morphology of chondrocytes was observed by optic
microscope. The DNA content was determined
by UV Spectrophotometry. The levels of IL-1beta and TNF-alpha in
cultured medium were measured by enzyme-linked
immunosorbent assay (ELISA).
RESULTS: Hematoxylin & eosin staining indicated there was cell
necrosis in the cartilage
reconstructed in vitro from both Nivalenol group and
Nivalenol+Se group. Compared
with the group of Nivalenol toxin, the damage of chondrocytes
was less severe when Se was added.
Nivalenol could inhibit chondrocyte DNA synthesis. The content
of DNA with Nivalenol was lowest than
that in other groups. The levels of IL-1beta and TNF-alpha
with Nivalenol were significantly higher than control
group (P<0.05). After Se was added,
the levels did not change significantly compared with the groups
without Se.
CONCLUSION: Nivalenol toxin could superinduce IL-1beta and
TNF-alpha secretion in chondrocytes,
which may be the key mechanism of chondrocyte injury by
Nivalenol . Se can partially alleviate
the effects of Nivalenol on chondrocytes cultured in vitro.
PMID: 20368105 |
