Ghatak C, Rao VG, Pramanik R, Sarkar S, Sarkar N.Nanocavity
Effect On Photophysical Properties Of Colchicine: A Proof by
Circular Dichroism Study and
Picosecond Time-Resolved Analysis in
Various Reverse Micellar Assemblies.
1. J Phys Chem B. 2011 May 4.
Department of Chemistry, Indian Institute of Technology ,
Kharagpur 721302, WB,
India.
In August 2009, colchicine won Food and Drug Administration
(FDA) approval in the United States as
a stand-alone drug for the treatment of acute flares of gout and familial Mediterranean fever. Recently, it is now the
center of attraction in medicinal
research. In this present paper, we have employed two other
analogues of colchicine for exploring
the photophysical properties inside nanocavity environment in details. Here we have a series of
interesting results that have
interesting similarity with the colchinoid-tubulin interaction.
To monitor fluorescence
properties of colchinoids, we have used absorption, emission,
and time-resolved spectroscopy
and to monitor structural properties we have measured
circular dichroism. Steady-state anisotropy and dynamic light
scattering results give an idea
about the microenvironment sensed by the colchinoids molecules.
A sharp increment for colchicine, very
small increment for isocolchicine and no increment for colcemid in fluorescence and different
circular dichroism (CD) spectra of all
of these colchinoids upon embedment inside nanocavity of reverse micelle made a supposition that all these changes of
fluorescence properties and CD
results of colchinoids is not solely due to viscosity effect but
also the constraint, that is, very
narrow space to spread over, given by the nanocavity of reverse micelle. Moreover, we have noticed that the B
ring of the colchinoids also have a
pronounced effect on the interaction nature as well as on conformational change of these compounds after
entrapment.
PMID: 21542580
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