|
Eur J Haematol. 2000 Jan;64(1):10-21. Links
Cell kinetic study of normal human bone marrow hematopoiesis and acute leukemia
using 7AAD / PY.
Toba K, Koike T, Watanabe K, Fuse I, Takahashi M, Hashimoto S, Takahashi H, Abe
T, Yano T, Shibazaki Y, Itoh H, Aizawa Y.
First Department of Internal Medicine, Niigata University School of Medicine,
Niigata City, Japan. tobaken@med.niigata-u.ac.jp
We have used the 7AAD /PY method to analyze the cell cycle status of normal
human bone marrow hematopoiesis, and found that the cell kinetics differed.
There were cells with relatively low levels of RNA in the S-phase (Type I) and a
high level in the S-phase (Type 11). T-cells, B-cells, nucleated red cells and
CD34+/CD19+ early B-cells in bone marrow were Type I, whereas myelomonocytic
subset and CD34+/CD33-dim+ common myeloid cells were Type II. AC133+/CD38-dim
cells, which were thought to be lineage-marker negative hematopoietic stem
cells, had intermediate amounts of RNA in the S-phase between Type I and II
(Type 0). Seventy-four cases of acute leukemia were also analyzed. Most of the
T- and B-ALL cases were found to be Type I, most of the ANLL cases were Type II,
and there were 10 cases that were Type 0. These findings yielded fundamental
information about normal hematopoiesis and acute leukemia.
PMID: 10680701
|
