| Source: | Semisynthetic.
Derived from Actinomycin D
(from
Streptomyces parvulus) |
| Synonyms | 7AAD,
7-AminoActinomycin D
|
| Description: |
7AAD is a semisynthetic derivate of Actinomycin D. 7aad binds to single
stranded DNA. |
| CAS number: | 7240-37-1 |
| Merck index: | 13, 2828 |
| Molecular weight: | 1270.4 |
| Structure: | 
 |
| Molecular Formula: |
C62H87N13O16 |
| Canonical SMILES |
CC1C(C(=O)NC(C(=O)N2CCCC2C(=O)N(CC(=O)N(C(C(=O)O1)C(C)C)C)C)C(C)C)NC(=O)C3=CC(=C(C4=C3N=C5C(=C(C(=O)
C(=C5O4)C)N)C(=O)NC6C(OC(=O)C(N(C(=O)CN(C(=O)C7CCCN7C(=O)C(NC6=O)C(C)C)C)C)C(C)C)C)C)N |
Solubility
information: |
Dichloromethane, DMSO, Methanol. Slightly soluble in water |
| Specifications |
Specification updated on 4 Sep. 2012;
Attention: the melting point and UV spectrum are brought here for
your information, but are not included into the product
specification |
| Appearance: | Purple powder |
| Purity: | At least 98% by TLC, HPLC |
λmax: |
|
| Melting point | 255°C-263°C |
| Solubility | 10 mg/ml of methanol gives a
clear red solution |
| Storage | -20°C. Protect from light.
Hygroscopic! |
| Applications | 7AAD (7-amino-Actinomycin D)
is an inhibitor of DNA-primed RNA polymerase and DNA polimerase.
7AAD (7-amino-Actinomycin D) induces apoptosis.
7AAD is used as a fluorescent
DNA Stain. Useful tool in determining apoptosis |
| Classification | oligopeptide antibiotic
RNA polymerase inhibitor |
| Related products |
Actinomycin D Chromomycin A3
|
| Warnings | TOXIC. CARCINOGENIC |
| | For Research use only. Not
for Human or Drug use
GMP/API grade available on request
7AAD is synthesized from
Actinomycin_D.
See there for raw material information
No genetically modified organisms are used. |
| Publications | |
| | Lecoeur H, Ledru E, Prevost MC,
Gougeon ML.
Strategies for phenotyping apoptotic peripheral human lymphocytes comparing
ISNT, annexin-V and 7-AAD cytofluorometric staining methods.
J
Immunol Methods. 1997 Dec 1;209(2):111-23. |
| | Toba K, Koike T, Watanabe K, Fuse
I, Takahashi M, Hashimoto S, Takahashi H, Abe T, Yano T, Shibazaki Y, Itoh
H, Aizawa Y.
Cell
kinetic study of normal human bone marrow hematopoiesis and acute leukemia
using 7AAD/PY.
Eur J Haematol. 2000 Jan;64(1):10-21. |
| | Indian J Exp Biol. 2006
Aug;44(8):640-6
Tri-iodo thyronine
regulates antioxidant enzyme activities in different cell fractions through
a mechanism sensitive to actinomycin D in a teleost, Anabas testudineus
(Bloch).
Saumya SM, Sreejith P, Vijayasree AS, Divya L, Manju M, Oommen OV. |
